Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

FluoroTect™ GreenLys in vitro Translation Labeling System

Gary Kobs, Robin Hurst, Natalie Betz and Becky Godat
Promega Corporation
Publication Date: 2001


The FluoroTect™ GreenLys in vitro Translation Labeling System is the first commercially available fluorescent technology for labeling in vitro translation products. The FluoroTect™ System uses a modified charged lysine transfer RNA (tRNA) labeled with the fluorophore, BODIPY®-FL. The modified lysine tRNA is used efficiently in both prokaryotic and eukaryotic in vitro translation systems. Data is obtained in a few minutes by the detection of the fluorescently labeled proteins "in-gel" with laser-based fluorescent gel scanning. This feature circumvents the multiple manipulations associated with traditional isotopic and nonisotopic incorporation and detection methodologies.

Promega Notes 77, 23–27.

Related Resources