Are the JM109 cells supplied with my vector competent? Are they already transformed?
*cfu or colony forming units is a standard unit of measurement for bacterial cells.
Must Promega plasmids be grown in the supplied JM109 cells?
We maintain most of our plasmids in JM109 cells, but there is no need to use only JM109. Most of our plasmids should grow in standard E. coli strains such as DH5α™ or XL-1 Blue. Exceptions to this include discontinued pALTER®-Ex1 and pALTER®-Ex2 Vectors which grow very slowly in DH5α™, and the discontinued pCAT® line of vectors, which are not stable in JM109 cells. If you have the discontinued pCAT® Vectors and wish to make more, use HB101 bacteria to propagate the plasmids.