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Using the QuantiFluor™ dsDNA System and the GloMax® Multi+ Fluorometer with Instinct™ Software

Abstract

Accurate quantitation of DNA concentration is critical for many applications. Traditional spectrophotometric assays are not practical for measuring DNA concentrations below 1ng/μl; however, many clinical and forensic samples have concentrations well below this level. The QuantiFluor™ dsDNA System is a fast, easy and sensitive method for determining low DNA concentrations. In this article, we have coupled the QuantiFluor™ dsDNA system with the GloMax® Multi+ fluorometer equipped with Instinct software allowing for instant DNA quantification by fitting the unknown sample’s signal to a calibration curve. This article will help guide the user through sample and calibration curve setup, GloMax® Multi+ Instrument setup and Instinct data analysis.

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