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RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods

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Abstract

In this report, we demonstrate the performance of two RNA isolation methods—one manual method (ReliaPrep™ RNA Cell Miniprep System) and one semi-automated method (Maxwell® 16 LEV simplyRNA Cells Kit)—for RNA extraction from 3D cultures developed using the InSphero hanging-drop method. The ReliaPrep™ RNA Miniprep Systems provide a fast and simple way to prepare intact total RNA from cultured cells or tissues in about 30 minutes. The column/binding matrix efficiently captures RNA from very small amounts of input material and the RNA is eluted in a minimal volume (less than 15µl). For the semi-automated method, we used the Maxwell® 16 LEV simplyRNA Cells Kit with the Maxwell® 16 Instrument. In this method, microtissue sample lysates are added directly to a prefilled reagent cartridge, which is then processed by the Maxwell® Instrument using an automated method.

Brad Hook and Mark Bratz

Promega Corporation

Publication Date: 9/14

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How to Cite This Article

Hook, B., and Bratz, M. RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods. [Internet] 9/14. [cited: year, month, date]. Available from: http://www.promega.com/resources/pubhub/applications-notes/rna-isolation-from-3d-microtissue-cultures/

Hook, B., and Bratz, M. RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods. Promega Corporation Web site. http://www.promega.com/resources/pubhub/applications-notes/rna-isolation-from-3d-microtissue-cultures/ Updated 9/14. Accessed Month Day, Year.