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Measuring GTPase, GEF and GAP Activities Using the GTPase-Glo™ Assay and the GloMax® Discover System

Promega Corporation
Publication Date: Feb 2016

Abstract

The sensitive GTPase-Glo™ Assay analyzes the intrinsic activities of GTPase, GAP-stimulated GTPase, GAP and GEF by enzymatic conversion of GTP remaining after the GTPase reaction into ATP followed by bioluminescent detection of the created ATP. Measuring the luminescence from the GTPase-Glo™ Assay is easy on the GloMax® Discover System because the protocol comes preloaded on the instrument. The extended dynamic range and minimal well-to-well cross talk of the GloMax® Discover System allows you to easily measure signals of varying intensities on the same plate. This Application Note describes the protocol to measure GAP-stimulated GTPase activity using the GTPase-Glo™ Assay and GloMax® Discover System.

Application Note #AN293

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

  Measuring GTPase, GEF and GAP Activities Using the GTPase-Glo™ Assay and the GloMax® Discover System. [Internet] Feb 2016. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/measuring-gtpase-gef-and-gap-activities-using-the-gtpase-glo-assay-and-the-glomax-discover-system/

American Medical Association, Manual of Style, 10th edition, 2007

  Measuring GTPase, GEF and GAP Activities Using the GTPase-Glo™ Assay and the GloMax® Discover System. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/measuring-gtpase-gef-and-gap-activities-using-the-gtpase-glo-assay-and-the-glomax-discover-system/ Updated Feb 2016. Accessed Month Day, Year.