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Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System

Promega Corporation
Publication Date: June 2017

Abstract

The Succinate-Glo™ Assay is a universal assay for detection of Fe(II)/α-KG-dependent hydroxylase and JumonjiC domain-containing histone lysine demethylase activities. The assay relies on enzymatic conversion of the succinate produced by the Fe(II)/α-KG-dependent hydroxylase reaction into ATP followed by bioluminescent detection of the created ATP. Measuring the luminescence from the Succinate-Glo™ Assay is easy on the GloMax® Discover System because the protocol comes preloaded on the instrument.

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Promega Corporation Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System. [Internet] June 2017. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/measure-jmjcdemethylasefeii-alpha-kg-hydroxylase-activitysuccinate-glo-assay-and-glomax-discover/

American Medical Association, Manual of Style, 10th edition, 2007

Promega Corporation Measuring JumonjiC Demethylase and Fe(II)/alpha-KG-Dependent Hydroxylase Activities Using the Succinate-Glo™ Assay and GloMax® Discover System. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/measure-jmjcdemethylasefeii-alpha-kg-hydroxylase-activitysuccinate-glo-assay-and-glomax-discover/ Updated June 2017. Accessed Month Day, Year.