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GloMax®-Multi Jr Method for Dual-Glo® Luciferase Assay System

Abstract

The GloMax®-Multi Jr Single-Tube Reader in combination with the Dual-Glo® Luciferase Assay System, enables fast, simple quantitation of firefly and Renilla luciferase activities in a single sample. Transcription regulation, coupled to the expression of a luciferase reporter gene, is regularly used to study a wide range of biological events in cultured cells. The benefit of using a dual-reporter system is that normalizing the expression of an experimental reporter to the expression of the control reporter can help differentiate between specific and nonspecific cellular responses. Firefly and Renilla luciferases are widely used as co-reporters for these normalized studies because the assays used to measure their activity are quick, easy and sensitive.