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RT-qPCR Using the PCR Optimization Kit

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Abstract

The PCR Optimization Kit provides a range of buffer formulations appropriate for endpoint and real-time PCR that can be used for manufacture of custom master mixes. With the addition of reverse transcriptase, RNase inhibitor and fluorescent dyes, these reagents also support RT-qPCR applications. This article shows how to use the buffers supplied in the PCR Optimization Kit to perform RT-qPCR and provides example functional data. 

Katharine D. Hoffmann and Leta S. Steffen

 

Promega Corporation

 

Publication Date: 7/18

Download Article (789 KB)

How to Cite This Article

Hoffmann, K.D., and Steffen, L.S.   RT-qPCR Using the PCR Optimization Kit. [Internet] 7/18. [cited: year, month, date]. Available from: http://www.promega.com/resources/pubhub/applications-notes/an300/rt-qpcr-using-the-pcr-optimization-kit/

Hoffmann, K.D., and Steffen, L.S.   RT-qPCR Using the PCR Optimization Kit. Promega Corporation Web site. http://www.promega.com/resources/pubhub/applications-notes/an300/rt-qpcr-using-the-pcr-optimization-kit/ Updated 7/18. Accessed Month Day, Year.