Pyruvate-Glo™ Assay Technical Manual

Instructions for Use of Product(s)
J4051, J4052

Literature # TM709

The Pyruvate-Glo™ Assay is a bioluminescent assay for rapid and sensitive detection of pyruvate. Pyruvate is a key energy metabolite that is an intermediate in various cell biochemical pathways, including the TCA cycle and glycolysis. The Pyruvate-Glo™ Assay is a two-step assay that involves an initial incubation with pyruvate oxidase and H2O2 Substrate, followed by addition of Pyruvate -Glo™ detection reagent. Results are available in less than 90 minutes. The assay is linear to 50μM pyruvate and sensitive to <800nM The result is a light signal proportional to the starting pyruvate concentration in the sample.

Summary of Changes
The following change was made to the 1/24 revision of this document: 
1. The volume of cells/well used in Figure 6 was changed to 100,000.

Revised 1/24.