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GeneMarker®HID Software for Spectrum CE Systems User Manual

Instructions for Use of Product(s)

Literature # TM555

GeneMarker®HID Software for Spectrum CE Systems (GMHID-Spectrum) is designed for analysis of short tandem repeat (STR) data generated by capillary electrophoresis (CE) systems, including the Spectrum CE Systems, as well as analysis of STR data from Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. GMHID-Spectrum allows genotyping using an allelic ladder and is optimized for human identification applications including forensic casework analysis, databasing, missing persons and relationship testing. GMHID-Spectrum provides a suite of integrated post-genotyping applications, including: expert system review, database creation and profile searching, contamination check, kinship analysis and population statistics calculation.

GMHID-Spectrum is compatible with commercial STR chemistries, including PowerPlex® and GenePrint® Systems from Promega and custom STR chemistries. The software supports CE instrument data files from numerous platforms, including Promega (.promega, .fsa) and Applied Biosystems (.fsa, .hid). GMHID-Spectrum is developed by SoftGenetics, LLC, and is sold and supported by Promega Corporation.

Summary of Changes:

The following changes were made to the 1/23 revision of this document:
1. Revised Sections 2.B and 2.C.
2. Updated images in Sections 4.A, 4.C and 8.A.
3. Inserted new Sections 4.C.6 Sample Quality Peak(s) and 10.A.4 Quality Indicators.
4. Updated Table 6 in Section 5.A.
5. Edited Sections 5.B.1, 5.E.2, 6.B.3, 6.B.4, 10.A and 13.
6. Inserted new Section 13.B Performing Stutter Ratio Calculations, and renumbered subsequent sections.
7. Moved document to a new template.

Revised 1/23.