ReliaPrep™ Large Volume HT gDNA Isolation System Technical Manual
Instructions for Use of Product(s)
A2751
Literature # TM341
The ReliaPrep™ Large Volume HT gDNA Isolation System isolates genomic DNA from 1ml to 10ml of blood in a scalable format. The chemistry eliminates tedious centrifugation steps as well as the use of hazardous chemicals, which are inherent in precipitation-based chemistries. The system can be automated on a robotic liquid-handling workstation, allowing walkaway purification of genomic DNA from 1–10ml whole blood, regardless of sample storage or shipping conditions.
Summary of Changes, 11/18 Revision
The following changes were made to the 11/18 revision of this document:
- On p. 2, the range of A260/A230 ratio was changed from “between 1.8 and 2.2” to “between 1.5 and 2.4” per a change in resin.
- Section 7, Troubleshooting was updated to add possible causes and solutions for cases of low DNA purity.
Printed in USA. Revised 11/18
Related Protocols
- Automated Genomic DNA Isolation Using the Tecan Freedom EVO-HSM Workstation Technical Manual
- Automated Genomic DNA Isolation from Whole Blood using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Buffy Coat Samples Using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Blood Cell Pellets using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Oragene Saliva Samples using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Mouthwash Samples using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Low Yielding Buffy Coat Samples using the Tecan Freedom EVO®-HSM Workstation
- Automated Genomic DNA Isolation from Low Volumes of Whole Blood using the Tecan Freedom EVO®-HSM Workstation
