PowerQuant® System Technical Manual
Literature # TMD047
The PowerQuant® System is a five-dye, four-target hydrolysis probe-based qPCR multiplex that amplifies multicopy targets to quantify the total human and human male DNA present in a sample. The system also amplifies an additional multicopy target to assess the degree of DNA degradation. Additionally, the PowerQuant® System includes an internal PCR control (IPC) to detect inhibitors in an amplification reaction.
This Technical Manual provides instructions for use with the Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6), Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2) or the QuantStudio™ 5 Real-Time PCR System.
Summary of Changes
The following changes were made to the 1/20 revision of this document:
1. Updated Figure 36 to correct a typo.
Printed in USA. Revised 1/20.