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PowerQuant® System Technical Manual

Instructions for Use of Product(s)
PQ5002, PQ5008

Literature # TMD047

The PowerQuant® System is a five-dye, four-target hydrolysis probe-based qPCR multiplex that amplifies multicopy targets to quantify the total human and human male DNA present in a sample. The system also amplifies an additional multicopy target to assess the degree of DNA degradation. Additionally, the PowerQuant® System includes an internal PCR control (IPC) to detect inhibitors in an amplification reaction.

This Technical Manual provides instructions for use with the Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6), Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2) or the QuantStudio™ 5 Real-Time PCR System.

Summary of Changes
The following changes were made to the 1/20 revision of this document:

1. Updated Figure 36 to correct a typo.

Printed in USA. Revised 1/20.

Experienced User Protocols