Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

PowerQuant® System Technical Manual

Instructions for Use of Product(s)
PQ5002, PQ5008

Literature # TMD047

The PowerQuant® System is a five-dye, four-target hydrolysis probe-based qPCR multiplex that amplifies multicopy targets to quantify the total human and human male DNA present in a sample. The system also amplifies an additional multicopy target to assess the degree of DNA degradation. Additionally, the PowerQuant® System includes an internal PCR control (IPC) to detect inhibitors in an amplification reaction.

This Technical Manual provides instructions for use with the Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6), Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2) or the QuantStudio™ 5 Real-Time PCR System.

Summary of Changes
The following changes were made to the 1/20 revision of this document:

1. Updated Figure 36 to correct a typo.

Printed in USA. Revised 1/20.

Experienced User Protocols