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HSM 2.0 Instrument Technical Manual

Instructions for Use of Product(s)
A2715

Literature # TM389

The Heater Shaker Magnet Instrument (HSM 2.0) is designed to perform all the functions necessary for processing magnetic resin-based purification chemistries in large-volume formats. By combining the ability to heat, shake and apply a magnetic field, the HSM 2.0 Instrument provides all-in-one processing capabilities for a variety of large-volume purification chemistries in either manual or automated formats. The instrument uses standard 50ml conical tubes and reagent-based paramagnetic particle (PMP) chemistries.
Initially designed to run the Promega ReliaPrep™ Large Volume HT gDNA Isolation System, the HSM 2.0 Instrument is supplied with software containing preprogrammed isolation methods for processing up to 32 samples of human whole blood in approximately 2–3.5 hours, depending on sample volume and number. Samples are processed in a semi-automated method with the user dispensing and aspirating reagents from the samples as directed by the software on a computer screen. The programmed methods control the shaking, heating, magnetization and timing of the steps required for the semi-automated purification. For fully automated purification, the HSM 2.0 Instrument can be integrated with a robotic liquid-handling workstation.

Summary of Changes:

The operating system requirements were updated in the 3/19 version of this document.

Printed in USA. Revised 3/19.

Experienced User Protocols