Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Wheat Germ Extract Technical Manual

Instructions for Use of Product(s)
L4380, L4330

Literature # TM230

Promega Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus minimize background translation. Wheat Germ Extract and Rabbit Reticulocyte Lysate contain the cellular components necessary for protein synthesis (tRNA, ribosomes and initiation, elongation and termination factors).

Only the addition of exogenous amino acids (including an appropriately labeled amino acid) and mRNA are necessary to stimulate translation. Potassium Acetate is supplied as a separate component so that the translational system may be optimized for a wide range of mRNAs.

Summary of Changes
The following change was made to the 1/19 revision of this document:
1. Corrected the amount of Luciferase Control RNA listed in Section 2, Product Components and Storage Conditions.

Printed in USA. Revised 1/19.

Experienced User Protocols