pRL Renilla Luciferase Reporter Vectors Protocol
Literature # TB550
The pRL Renilla Luciferase Reporter Vectors are intended for use as an internal control reporters in combination with any experimental reporter vector to cotransfect mammalian cells. All of the pRL Reporter Vectors contain cDNA encoding Renilla luciferase (Rluc), which was originally cloned from the marine organism Renilla reniformis (sea pansy). Renilla luciferase is a 36kDa monomeric protein that does not require post-translational modification for activity. Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. The Renilla luciferase cDNA contained within the pRL Reporter Vectors has been modified slightly to provide greater utility.
The pRL-CMV, pRL-SV40 and pRL-TK Vectors contain promoters or enhancers or both to express Renilla luciferase in cotransfected mammalian cells. The pRL-null Vector lacks promoter and enhancer elements and is intended for use in constructing a control reporter vector for expression in mammalian cells with an experimental reporter vector.
Printed in USA. Revised 12/08.