Instructions for Use of Product(s)
Literature # TBI001
The ISOQUANT® Isoaspartate Detection Kit is intended for quantitative detection of isoaspartic acid residues in proteins and peptides, which can result from the gradual, nonenzymatic deamidation of asparagine or rearrangement of aspartic acid residues during storage or handling.
The ISOQUANT® Kit provides reagents for deamidation detection using an HPLC method. The amount of isoaspartic acid to target protein is determined by comparison with reactions performed using a reference standard peptide.
Summary of Changes, 6/17 Revision
The following changes were made to the 6/2017 revision of this Technical Bulletin:
1. In Section 3.D., page 10, Step 5 was added, and Step 6 was modified to include a centrifugation speed.
2. In Section 3.E., Step 3 was revised to recommend use of a temperature-controlled autosampler set at 4°C.
Printed in USA. Revised 6/17.
Experienced User Protocols