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Wizard® SV 9600 Plasmid DNA Purification System Technical Bulletin

Instructions for Use of Product(s)

Literature # TB292

This system requires the use of the Vac-Man® 96 Vacuum Manifold or compatible vacuum manifold. Plasmid DNA is purified from bacterial lysates using a 96-well vacuum filtration step to simultaneously clear the bacterial lysate and bind plasmid DNA, eliminating the need for centrifugation. Removal of the Lysate Clearing Plate and subsequent washing of the bound plasmid DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection vessels during plasmid DNA recovery. DNA is collected by elution into a 96-well plate.

Printed in USA. Revised 1/12.