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pGEM®-5Zf(+) Vector Technical Bulletin

Instructions for Use of Product(s)

Literature # TB047

The pGEM®-5Zf(+) Vector is a derivative of the pGEM®-3Zf(+) Vector. The plasmid serves as a standard cloning vector and as a template for in vitro transcription. pGEM®-5Zf(+) contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates. The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI. The polylinker contains restriction enzyme sites that produce 5´ overhangs or blunt ends (sensitive to exonuclease III), flanked on both sides by blocks of restriction sites that generate 3´ overhangs (resistant to exonuclease III).

Printed in USA. Revised 4/17.