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HaloCHIP™ System

Covalent Capture of Protein:DNA Complexes, No Antibodies Needed

  • No need to make or purchase qualified antibodies
  • Results in 24–48 hours with fewer steps, minimizing potential errors

Size

Catalog number selected: G9410

$ 584.00 Your price: Log In

HaloCHIP™ System
20 reactions
$ 584.00
Your price: Log In

Covalent Protein:DNA Complex Capture

The HaloCHIP™ System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies.  HaloCHIP™ System offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method. Proteins of interest are expressed in cells as HaloTag® fusion proteins, crosslinked to DNA with formaldehyde and then captured on HaloLink™ Resin, which forms a highly specific, covalent interaction with the HaloTag® portion of the fusion protein. Stringent washing removes nonspecific proteins and DNA, and heating reverses the crosslinks between the DNA and the fusion protein and releases the captured DNA fragment, which subsequently can be purified.

The HaloCHIP™ System helps you obtain data in 24–48 hours with fewer steps, minimizing potential experimental errors, and with improved signal-to-noise ratios, to detect small changes in protein-binding from a minimal number of cells.

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology Products page.

Application
  • Protein:DNA interaction analysis.
6633MB.eps
Representation of the HaloCHIP™ System.

Protocols

Video Protocols

halochip01-fallback

Specifications

What's in the box?

Item Part # SizeAvailable Separately

HaloLink™ Resin

G191A 1 × 0.5ml

Mammalian Lysis Buffer

G938A 1 × 40ml View Product

High Salt Wash Buffer

G939A 1 × 25ml

Reversal Buffer

G940A 1 × 8ml

HaloCHIP™ Blocking Ligand

G5491 1 × 30μl

Nuclease-Free Water

P1195 1 × 150ml

TE Buffer, 1X, Molecular Biology Grade

V6231 1 × 100ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,429,472, 7,888,086, 8,202,700 and other patents pending.

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