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NanoBRET™ Protein Interaction Assays

We have developed NanoBRET™ Protein:Protein Interaction (PPI) Assays to measure the interaction of specific protein pairs for a variety of biological targets. Assays are available by direct order from our catalog or by custom order. Click on the catalog number to visit the product catalog page or check the box next to assays of interest to submit an inquiry to our Custom Assay Services team for more information.

Custom materials are developed by the Custom Assay Services team within R&D. Although functionally tested, they are not manufactured under the Promega ISO13485 quality system and are sold "as is" with no warranty either stated or implied.

  • Signaling Proteins and Kinase Assays

    Name
    β-catenin/BCL9L
    β-catenin/TCF4
    bRaf/cRaf
    cRaf/cRaf (Dimerization)
    FKBP/FRB
    KRas(G12C)/bRaf FL and RBD
    KRas(G12D)/bRaf FL and RBD
    KRas(WT)/bRaf FL and RBD
    MAVS/IFIH1
    MID1/Alpha4
    SHN3 mut/ERK1
    SHN3 WT ERK1
    SKP2/CKS1B
    Name
    β-catenin/TCF3
    bRaf/bRaf (Dimerization)
    cMyc/AURKA
    ERK/ELK
    GADD45B/MKK7
    KRas(G12C)/cRaf FL and RBD
    KRas(G12D)/cRaf FL and RBD
    KRas (WT)/cRaf FL and RBD
    MEK/ERK
    MycN/AURKA
    SHN3 mut/ERK2
    SHN3 WT/ERK2
  • Other Epigenetic Assays

    Name
    CBX2/H3.1
    CBX7/Histone H3.2
    EZH2/SUZ12
    HDAC1/HDAC2
    MLL1/Menin
    PRMT5/RIOK1
    TUDOR/Histone H3.3
    Name
    CBX7/Histone H3.1
    CBX7/Histone H3.3
    FANCD2/Ubiquitin
    KDM4B/Histone H3.3
    MLL1/WDR5
    PRMT5/WDR77(MEP50)
  • Transcriptional Protein Assays

    NameCatalog #
    NanoBRET™ cMyc/MAX Interaction AssayN1870
    NanoBRET™ PPI Control Pair (p53/MDM2)N1641


    Name
    cMYc/WRD5
    FOXP3/FOXP3
    GR/GR (Dimerization)
    IRF5 Iso1 2D Dimer
    IRF5 WT Isoform 2 Dimer
    IRF5-6A mutant Dimer
    TEAD1/TAZ
    TEAD2/TAZ
    TEAD3/TAZ
    TEAD4/TAZ
    Name
    ER/ER(Dimerization)
    FOXP3/NFATC2
    IRF5/IRF5 Dimer
    IRF5 Iso16D Dimer
    IRF5-2A mutant Dimer
    SP140/Histone H3.3
    TEAD1/YAP1
    TEAD2/YAP1
    TEAD3/YAP1
    TEAD4/YAP1
  • Membrane Protein Assays

    Name
    DAP12/Syk
    GRB2/EGFR
    IL17RA/ACT1
    MERTK dimer
    Name
    DAP12/TREM2
    HCN1/TRIP8b
    MET/Grb2
    STIM1/Orai
  • Cellular Regulation

    Name
    ABHD5/PLIN5
    MCL-1/BIML
    Name
    CALR/MPL
    PNPLA3/ABHD5
  • Protein Degradation

    Name
    BRD4/CRBN
    Name
    E6/E6AP
  • Bromodomain Assays

    NameCatalog #
    NanoBRET™ BRD 4/Histone H3.3 Interaction AssayN1830


    Name
    ATAD2/Histone H3.3
    BRD2/Histone H3.3
    BRD3/Histone H3.3
    BRD4 BD2/Histone H3.3
    BRD7 BD/Histone H3.3
    CBP/Histone H4
    EP300 BD/Histone H4.4
    Name
    ATAD2/Histone H4
    BRD2/Histone H4
    BRD3/Histone H4
    BRD4 BD2/Histone H4
    CBP/H3.3
    EP300/Histone H3.3
    SMARCA2/Histone H3.3
Name
Address