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HaloTag® Ligand Building Blocks

Enables any Reporter, Protein or Nucleic Acid with Specified Functional Groups to be used with the HaloTag® Protein

  • Image live or fixed mammalian cells expressing HaloTag® protein or protein fusions
  • Analyze post-translational modification
  • Isolate proteins and protein complexes
  • Cat.# P1681 and P6761 are discontinued.

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Catalog number selected: P6711

$ 554.00 Your price: Log in

HaloTag® Ligand Building Blocks
Amine (O2)/5mg
$ 554.00
Your price: Log in
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Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.

The HaloTag® Succinimidyl Ester (O4) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (O4). The HaloTag® Succinimidyl Ester (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag® Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages (see figure). The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Amine (O4) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (O4). The HaloTag® Amine (O4) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag® Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters (see figure), STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Iodoacetamide (O4) Ligand contains a reactive iodoacetamide group connected an alkyl chloride separated by an ethylene glycol repeat (O4). The HaloTag® Iodoacetamide (O4) Ligand has been designed to rapidly react with sulfhydryl-containing molecules (see figure), whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Iodoacetamide (O2) Ligand contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat (O2). HaloTag® Iodoacetamide (O2) Ligand has been designed to rapidly react with sulfhydryl-containing molecules (see figure), whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag® protein for any application of interest.

The HaloTag® Thiol (O4) Ligand contains a reactive sulfhydryl group connected to an alkyl chloride separated by three ethylene glycol repeats (O4). The HaloTag® Thiol (O4) Ligand can be successfully conjugated to any reporter group, cross-linking reagent (bound or free), or nucleic acid derivative containing a number of different alkylating groups, forming stable thioether bonds. Commonly used reagents (see figure) that rapidly react with sulfhydryls include iodo- or bromo-acetyls or benzyls, bromo- or chloro-mustards, maleimides, aziridines, acryloyl derivatives, and halide or sulfonate containing arenes (those bearing Electron Withdrawing Groups (EWGs) react most rapidly). The reactive ligand can be captured with HaloTag® protein either before or after the thiol group is functionalized for any application of interest.

For more information, see the Protocols & Applications Guide.

References
  1. Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 139–40.
  2. Gretch, D.R., Suter, M. and Stinski, M.F. (1987) Anal. Biochem. 163, 270–77.
  3. Duthie, R.S. et al. (2002) Bioconjugate Chem. 13, 699–706.
  4. Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 137–46.
  5. Gee, K.R. et al. (1999) Tetrahedron Letters 40, 1471–4.
  6. Adamczyk, M. et al. (1997) Bioconjugate Chem. 8, 253–5.
  7. Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–8.
  8. Chen, J. and Selvin, P.R. (1999) Bioconjugate Chem. 10, 311–5.
  9. Ge, P. and Selvin, P.R. (2003) Bioconjugate Chem. 14, 870–76.
  10. Krokhin, O.V. et al. (2003) Biochemistry 42, 12950–59.
  11. Hermanson, G.T. (1996) In: Bioconjugate Techniques, Academic Press, San Diego, CA, 146–52.
6081MA.eps
Example reaction with HaloTag® Amine (O4) Ligand.

Specifications

You are viewing: P6711 Change Configuration

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: P6741 Change Configuration

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: P1681 Change Configuration

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Specifications

You are viewing: P6771 Change Configuration

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: P1691 Change Configuration

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: P6751 Change Configuration

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may modify or link Promega HaloTag® ligand building blocks to Promega or customer-supplied moieties. No transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: P6761 Change Configuration

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

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