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HaloTag® Custom Vectors and Ligands

HaloTag® technology is a comprehensive platform for functional protein analysis based on the formation of a covalent bond between a protein fusion tag (HaloTag) and synthetic chemical ligands. By using different ligands you can control the function and properties of the HaloTag® fusion protein. The HaloTag® system gives exceptional performance in cell-based and biochemical assays without the need to switch fusion tags or reclone inserts.

In addition to the HaloTag® Vectors and Ligands available as catalog items, we offer a selection of pre-built custom HaloTag® vectors and ligands developed by our Custom Assay Services team within R&D. Although functionally tested, they are not manufactured under the Promega ISO13485 quality system and are sold “as is” with no warranty either stated or implied.

HaloTag® Vectors

N-terminal and C-terminal HaloTag® Flexi® Vectors for attenuated expression, protein secretion and nuclear localization in mammalian cells, and vectors containing regulated mammalian, TK, SV40, PGK, EF1α promoters are available.

View HaloTag® Vectors

HaloTag® Ligands

  • pH sensor ligand to detect internalization of membrane proteins
  • Eosin ligand for chromophore-assisted laser inactivation (CALI) for targeted protein destruction
  • HaloTag® PROTAC3 (proteolysis targeting chimera) is a permeable small-molecule compound that can target HaloTag® fusion proteins for degradation. HaloTag® PROTAC will bind covalently to HaloTag and induce interactions with E3 ligase machinery proteins, resulting in ubiquitination of the HaloTag® fusion and subsequent trafficking to the proteasome for degradation.
View HaloTag® Ligands

How Can We Help You?

If you don’t see what you are looking for in the lists above or in our catalog listing, please contact Custom Assay Services to discuss your needs further. Custom Assay Services offered by Promega include:

  • Cell Engineering: Target expression.
  • Assay Development and Qualification: Target class expertise and multiple assay formats.
  • Assay-Ready Cells In-Scale: Functionally tested.