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Anti-NanoLuc® Monoclonal Antibody

Detects NanoLuc® Luciferase and NanoLuc® Fusion Proteins

  • Affinity-purified mouse monoclonal antibody
  • Use in Western blotting

Size

Catalog number selected: N7000

$ 349.00
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This product is discontinued
Anti-NanoLuc® Monoclonal Antibody
100μg
$ 349.00
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Antibody to Detect NanoLuc® Luciferase in Western Blot Applications

Anti-NanoLuc® Monoclonal Antibody can be used to detect NanoLuc® Luciferase or NanoLuc® fusion proteins by Western blotting. The Anti-NanoLuc® Antibody is a protein A/G affinity-purified mouse monoclonal antibody. 

For Western blotting, we recommend a concentration of 1µg/ml as a starting point for protocol optimization.

NanoLuc® luciferase is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. The small size and bright signal make NanoLuc® a versatile reporter that is used in many protein analysis applications. NanoLuc® fusion proteins are used for protein stability analysis and in NanoBRET™ target engagement and protein interaction applications. 

Western Blot Results
The figure on the right shows an example Western blot using HEK293 cells transfected with CMV-based expression constructs encoding NanoLuc® fusion proteins. The actual mobility approximated the expected mobility for each fusion protein. Background bands are evident in all lanes, including HEK293 cell lysate, which lacks expression of a NanoLuc® fusion protein, but the NanoLuc® fusion bands are more prominent.

Detection of NanoLuc Luciferase on Western Blot with Anti-NanoLuc Antibody

Detection of NanoLuc® fusion proteins expressed in HEK293 cells.

Specifications

What's in the box?

Item Part # Size

Anti-NanoLuc® Monoclonal Antibody

N700A 1 × 100μg

SDS

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Download SDSPDF (191 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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