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Save 20% on GloMax® Instruments until December 31st, 2023 View Details ›

Anti-NanoLuc® Monoclonal Antibody

Detects NanoLuc® Luciferase and NanoLuc® Fusion Proteins

  • Affinity-purified mouse monoclonal antibody
  • Use in Western blotting and immunofluorescence


Catalog number selected: N7000

$ 379.00
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Anti-NanoLuc® Monoclonal Antibody
$ 379.00
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Antibody to Detect NanoLuc® Luciferase

Anti-NanoLuc® Monoclonal Antibody can be used to detect NanoLuc® Luciferase or NanoLuc® fusion proteins by Western blotting and immunofluorescence. The Anti-NanoLuc® Antibody is a protein A/G affinity-purified mouse monoclonal antibody.

NanoLuc® luciferase is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. The small size and bright signal make NanoLuc® a versatile reporter that is used in many protein analysis applications. NanoLuc® fusion proteins are used for protein stability analysis and in NanoBRET™ target engagement and protein interaction applications.

Western Blot Results
The figure on the right shows an example Western blot using HEK293 cells transfected with CMV-based expression constructs encoding NanoLuc® fusion proteins. The actual mobility approximated the expected mobility for each fusion protein. For Western blotting, we recommend a concentration of 1µg/ml as a starting point for protocol optimization. Background bands are evident in all lanes, including HEK293 cell lysate, which lacks expression of a NanoLuc® fusion protein, but the NanoLuc® fusion bands are more prominent.

Detection of NanoLuc Luciferase on Western Blot with Anti-NanoLuc Antibody

Detection of NanoLuc® fusion proteins expressed in HEK293 cells.

Immunofluorescence Results
Shown below are example immunofluorescent images of HeLa cells transfected with a CMV-based construct expressing CDK2-NanoLuc® fusion protein. The CDK2-NanoLuc® fusion protein is stained red, and nuclei are marked blue via Hoechst staining. We recommend a concentration of 1-7.5µg/ml for immunofluorescence optimization. No NanoLuc® staining is observed in HeLa cells transfected with only carrier DNA (mock).

(3µg/ml Anti-NanoLuc®)


Mock (5µg/ml Anti-NanoLuc®)



What's in the box?

Item Part # Size

Anti-NanoLuc® Monoclonal Antibody

N700A 1 × 100μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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