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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

M1701

High Yields of cDNA from Long RNA Templates (>5kb)

Size

Catalog number selected: M3681

$ 41.00 Your price: Log In

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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
2,500u
$ 41.00
Your price: Log In
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Obtain Higher Yields of cDNA than with the Deletion Mutant

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Advantages

  • RNase H Minus: Provides optimal conditions to prepare full-length cDNA from long RNA templates.
  • Temperature Stability: Thermostability of this point mutant minimizes problems associated with secondary structure.
  • Increased Polymerase Activity: M-MLV RT (H–), Point Mutant, gives higher yields of cDNA compared with the deletion mutant (Cat.# M5301).
  • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
  • Broad Working Range: More tolerance to variations in enzyme and substrate concentrations means improved consistency in performance.
Applications
  • First-strand cDNA synthesis.
  • Primer extension.
11143TA.eps
Thermal stability and synthesis of large transcripts.

Protocols

Specifications

You are viewing: 2,500u Change Configuration

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Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

M368A 1 x 2,500 units

M-MLV RT 5X Buffer

M531A 1 x 1ml

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 10,000u Change Configuration

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Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

M368B 1 x 10,000 units

M-MLV RT 5X Buffer

M531A 1 x 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: 50,000u Change Configuration

What's in the box?

Item Part # Size

M-MLV RT, RNase (H–), Point Mutant

M368C 1 x 50,000 units

M-MLV RT 5X Buffer

M531A 5 x 1ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

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