Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

SV Total RNA Isolation System Protocol

Instructions for Use of Product(s)
Z3100, Z3101, Z3105

Literature # TM048

The SV Total RNA Isolation System provides a fast and simple technique for preparing purified and intact total RNA from tissues, cultured cells and white blood cells in as little as one hour, depending on the number of samples to be processed. Like the Wizard® Plus SV DNA Purification System (Cat.# A1330), either a spin or vacuum ("SV") purification protocol can be used. Up to 60mg of tissue can be processed per purification, depending on the type, function and RNA expression levels of the tissue. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation.

Summary of Changes
The following changes were made to the 7/18 revision of this document:
1. A typographical error was corrected.

Printed in USA. Revised 7/18.

Experienced User Protocols