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Save 20% on ReliaPrep™️ DNA & RNA Clean-Up and Concentration Systems through December 31st. Shop Now › 

Wizard® Plus Megapreps DNA Purification System

More than 1mg Plasmid from 1L Cultures

  • 3-hour protocol
  • Resin-based batch column method
  • No organic extractions

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Catalog number selected: A7300

$ 201.00
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Wizard® Plus Megapreps DNA Purification System
5 preps
$ 201.00
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The Wizard® Plus Megapreps DNA Purification System provides a rapid method for large-scale purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. Plasmid DNA yields more than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Wizard® Plus Megapreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Cell Resuspension Solution

A711C 1 × 150ml View Product

Cell Lysis Solution

A712D 1 × 150ml View Product

Neutralization Solution

A713C 2 × 150ml View Product

Maxi/Megapreps Filtering System

A742D 1 × 5 pack View Product

Wizard® Megapreps DNA Purification Resin

A768A 1 × 100ml View Product

Column Wash Solution

A810C 1 × 125ml View Product

SDS

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Download SDSPDF (188 KB) – English (United States)

Certificate of Analysis

See all certificates for A7300

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 5,808,041.

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