PureYield™ Plasmid Midiprep Start-Up Kit

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Plasmid Midiprep System, Vacuum Manifold and Free Vacuum Pump

  • Up to 200µg of plasmid from 50ml culture
  • Transfection-quality plasmid DNA in 30 minutes
  • No tedious, gravity-drip columns or post-elution alcohol precipitation
  • For additional voltage options, contact Technical Service

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Catalog number selected: A6740

$ 1,558.00
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PureYield™ Plasmid Midiprep Start-Up Kit
1 each
$ 1,558.00
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Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Midiprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Midiprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.

The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.

The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.

PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.


What's in the box?

Item Part # Size Available Separately

Eluator™ Vacuum Elution Device

A1071 1 × 4 each View Product

PureYield™ Plasmid Midiprep System

A2495 1 × 100 preps

Welch Vacuum Pump for North America Electrical

A6720 1 × 1 each

Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

A7231 1 × 1 each View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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