MyGlo Instrument now $4950. Use code GETMYGLO at checkout to get 10% off MyGlo. Shop Now ›

pGL3 Luciferase Reporter Vectors

E1741_pGL3-Control-Vector--20ug_3
customize-this-small
View information on global supply logistics

We are sorry, but these products are discontinued.

These plasmids are now available at Addgene.

For more information, contact Technical Service.

Please Enquire
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
pGL3 Luciferase Reporter Vectors

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis- or trans-acting factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, with a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.

For the most advanced reporter vectors and widest selection of features, please see the pGL4 Luciferase Reporter Vectors.

The specific transcriptional characteristics of the pGL3 Vectors will vary for different cell types. This may be particularly true for COS cells, which contain the SV40 large T antigen that promotes replication from the SV40 origin found in the promoter of the pGL3-Promoter and pGL3-Control Vectors. The combination of the large T antigen and the SV40 origin will result in a higher copy number of these vectors in COS cells, which in turn may result in increased expression of the reporter gene compared to other cell and vector combinations.

The pGL3 Luciferase Reporter Vectors offer different combinations of the SV40 early promoter and enhancer sequences to provide flexible experimental strategies and user convenience. Functional mapping of genetic elements can be achieved through nested deletions, and single-stranded DNA may be prepared for mutagenesis or sequencing. Primers are available for sequencing across cloning sites and for primer extension analysis.

0746VA08_4A-W

pGL3-Basic Vector GenBank® Accession Number U47295 and vector sequence text file.

0747VA08_4A-W

pGL3-Control Vector GenBank® Accession Number U47296 and vector sequence text file.

0745VA08_4A-W

pGL3-Enhancer Vector GenBank® Accession Number U47297 and vector sequence text file.

0748VA08_4A-W

pGL3-Promoter Vector GenBank® Accession Number U47298 and vector sequence text file.

Specifications

You are viewing: E1751 Change Configuration

What's in the box?

Item Part # Size Concentration

pGL3-Basic Vector

E175A 1 × 20μg 1μg/μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1741 Change Configuration

What's in the box?

Item Part # Size Concentration

pGL3-Control Vector

E174A 1 × 20μg 1μg/μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1771 Change Configuration

What's in the box?

Item Part # Size

pGL3-Enhancer Vector

E177A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1761 Change Configuration

What's in the box?

Item Part # Size Concentration

pGL3-Promoter Vector

E176A 1 × 20μg 1μg/μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

Chris

Chris

US