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GloResponse™ NFAT-RE-luc2P HEK293 Cell Line

GloResponse™ NFAT-RE-luc2P HEK293 cells response to PMA titration.

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Luciferase Reporter Cell Line with NFAT Response Element

  • Rapid, convenient analysis of any cellular response resulting in modulation of NFAT activities


Catalog number selected: E8510

$ 12,849.00
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GloResponse™ NFAT-RE-luc2P HEK293 Cell Line
2 vials
$ 12,849.00
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The GloResponse™ NFAT-RE-luc2P HEK293 Cell Line is a clonal derivative of Human Embryonic Kidney 293 (HEK293) cells. These cells contain a luciferase gene (luc2P) under the control of a minimal TATA promoter with multiple Nuclear Factor of Activated T-cell response elements (NFAT-REs). NFAT-REs are the DNA-binding sequences for the NFAT family of transcription factors, which are responsible for regulating a variety of biological functions including cell proliferation, cytokine production, and cardiovascular development. The GloResponse™ NFAT-RE-luc2P HEK293 Cell Line is designed for rapid and convenient analysis of any cellular response that results in modulation of NFAT activities.

GPCRs regulate a wide-range of biological functions and are one of the most important target classes for drug discovery. GPCR signaling pathways can be categorized into three classes based on the G protein α-subunit involved: Gs, Gi/o and Gq. The GloResponse™ NFAT-RE-luc2P HEK293 Cell Line can be used for Gq-coupled GPCRs, which signal through calcium ion release and activate the Nuclear Factor of Activated T-Cells response element (NFAT-RE).

The GloResponse™ Cell Lines incorporate the improvements developed for the pGL4 family of reporter vectors for enhanced performance. The destabilized luc2P luciferase reporter is used for improved responsiveness to transcriptional dynamics. The luc2P gene is codon optimized for enhanced expression in mammalian cells, and the pGL4 plasmid backbone was engineered to reduce background reporter expression. The result is a cell line with very high induction levels when the pathway of interest is activated.

GloResponse NFAT-RE-luc2P HEK293 cells response to PMA titration.

GloResponse™ NFAT-RE-luc2P HEK293 cells response to PMA titration. A total of 10,000 GloResponse™ NFAT-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and threefold serial dilutions of PMA were added to induce reporter gene expression. After 16 hours of induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.


What's in the box?

Item Part # Size

GloResponse™ NFAT-RE-luc2P HEK293 Cell Line

E851A 2 × 1 vial

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

Patent Pending.

U.S. Pat. No. 8,008,006.

Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.

HEK293 cells were obtained under license from AdVec Inc.



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