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Wizard® Plus Megapreps DNA Purification System

A7300

More than 1mg Plasmid from 1L Cultures

  • 3-hour protocol
  • Resin-based batch column method
  • No organic extractions

Size

Catalog number selected: A7300

$ 187.00 Your price: Log In

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Wizard® Plus Megapreps DNA Purification System
5 preps
$ 187.00
Your price: Log In

The Wizard® Plus Megapreps DNA Purification System provides a rapid method for large-scale purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. Plasmid DNA yields more than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Wizard® Plus Megapreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Cell Resuspension Solution

A711C 1 x 150ml View Product

Cell Lysis Solution

A712D 1 x 150ml View Product

Neutralization Solution

A713C 2 x 150ml View Product

Maxi/Megapreps Filtering System

A742D 1 x 5 pack View Product

Wizard® Megapreps DNA Purification Resin

A768A 1 x 100ml View Product

Column Wash Solution

A810C 1 x 125ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 5,808,041.

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