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Wizard® SV Genomic DNA Purification System

Extracts DNA from Tissues or Cells

  • Isolate DNA from cultured cells in 20 minutes
  • Choice of spin or vacuum purification protocols

Size

Catalog number selected: A2360

$ 124.00 Your price: Log In

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Wizard® SV Genomic DNA Purification System
50 preps
$ 124.00
Your price: Log In
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Simple DNA Purification Method for Cells and Tissues

The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.

The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).

Sample Types Processed With Wizard® SV Genomic

Blood
Sample TypePromegaExternalComments
Whole blood
Yes
Researchers isolated bacterial DNA from whole blood samples inoculated with Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. Smith, K., Diggle, M.A. and Clarke, S.C. (2003) J. Clin. Microbiol. 41, 2440–3.
PubMed ID #12791861.
Peripheral blood mononuclear cells
Yes
Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705.
PubMed ID #16361556.
Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes
Yes
Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970.
Mammalian Tissues
Sample TypePromegaExternalComments
Mouse tail clipping
Yes
Yes
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Reference:
Smith, P. et al. (2005) J. Exp. Med. 202, 1319–25.
PubMed ID #16301741.
Mouse liver
Yes
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse brain
Yes
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse heart
Yes
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Mouse spleen
Yes
Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42.
PubMed ID #16498502.
Mouse ear
Yes
Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012.
Mouse skin
Yes
Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14.
PubMed ID #16818633.
Anopheles funestus mosquito carcass
Yes
Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581.
Human lung cancer biopsy
Yes
Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743.
Human distant normal colorectal mucosa and tumor tissue
Yes
Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994.
Human frozen thymic epithelial tumor samples
Yes
Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6.
PubMed ID #16762968.
Human pancreatic islet
Yes
Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849.
Cultured Cells
Sample TypePromegaExternalComments
Cultured cells (Established cell lines are not listed.)
Yes
A protocol for DNA isolation from mammalian cultured cells is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. Any additional resources are listed below.
Chicken retinospheroid cultures
Yes
Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24.
PubMed ID #16207942.
HT1080 human fibrosarcoma
Yes
Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80.
PubMed ID #16973543.
Monkey primary fibroblasts, virus-infected
Yes
Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72.
PubMed ID #16809302.
Bacteria
Sample TypePromegaExternalComments
Bacillus macyae
Yes
Cells in mid- to late exponential phase were centrifuged for 20 minutes at 21,000 × g at 4°C.
Santini, J.M. et al. (2004) Int. J. Syst. Evol. Microbiol. 54, 2241–4.
PubMed ID #15545465.
E. coli K-12 derivative, MC4100
Yes
Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21.
PubMed ID #13129953.
Pseudomonas aeruginosa
Yes
Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903.
PubMed ID #15161855.
Bacillus subtilis
Yes
Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012.
Eleven Gram-negative bacterial species
Yes
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488.
Eleven Gram-positive bacterial species
Yes
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488.
Other Organisms and Tissues
Sample TypePromegaExternalComments
Schistosoma mansoni (blood fluke) worm
Yes
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716.
Snail cercariae
Yes
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716.

Protocols

Specifications

You are viewing: A2360 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Wizard® SV Minicolumns

A129A 1 × 50/pk

Collection Tubes

A130A 1 × 50/pk

Column Wash Solution (CWA)

A1311 1 × 185ml

Nuclei Lysis Solution

A7941 1 × 50ml

RNase A Solution

A797A 1 × 250μl View Product

Nuclease-Free Water

P119C 2 × 25ml View Product

EDTA, 0.5M (pH 8.0)

V423B 1 × 30ml View Product

Wizard® SV Lysis Buffer

Z3052 1 × 50ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2361 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Wizard® SV Minicolumns

A129B 1 × 250/pk

Collection Tubes

A130B 1 × 250/pk

Column Wash Solution (CWA)

A1311 2 × 185ml

Nuclei Lysis Solution

A7941 1 × 50ml

RNase A Solution

A797C 2 × 1ml4mg/ml View Product

Nuclease-Free Water

P1195 1 × 150ml

EDTA, 0.5M (pH 8.0)

V423B 1 × 30ml View Product

Wizard® SV Lysis Buffer

Z3052 2 × 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

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