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SacII

  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

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Catalog number selected: R6221

$ 74.00
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SacII
10u/µl/500u
$ 74.00
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Cut Site:

CC   GCGG
GGCG   CC

Incubation Conditions: Buffer C. 37°C.

Source: Streptomyces achromogenes.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™
100%* 50–75% 100% 50–75% <10%

*Buffer A is not recommended for use with supercoiled plasmid due to incomplete cutting.

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
4 33 1 0 0 0

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Buffer C 10X Buffer

R003A 1 × 1ml

Bovine Serum Albumin, Acetylated

R396D 1 × 150μl10mg/ml View Product

SacII

R622A 1 × 500u

SDS

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Download SDSPDF (91 KB) – English (United States)

Certificate of Analysis

See all certificates for R6221

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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Manas

Manas

India