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MULTI-CORE™ Buffer Pack

Convenient Aliquots of the Promega Universal Restriction Enzyme 10X Buffer

  • Enables digestion of DNA with more than one enzyme in a single reaction
  • Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions

Size

Catalog number selected: R9991

$ 13.00
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MULTI-CORE™ Buffer Pack
3 × 1ml
$ 13.00
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The MULTI-CORE™ Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE™ Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE™ Buffer.

See the table of Promega 1X Restriction Enzyme Buffer Composition below.

1X Restriction Enzyme Buffer Composition.

Buffer pH (at 37°C) Tris-HCl (mM) MgCl2 (mM) NaCl (mM) KCl (mM) DTT (mM)
A 7.5 6 6 6 1
B 7.5 6 6 50 1
C 7.9 10 10 50 1
D 7.9 6 6 1,500 1
E 7.5 6 6 100 1
F 8.5 10 10 100 1
G 8.2 50 5
H 7.5 90 10 50
J 7.5 10 7 50 1
K 7.4 10 10 150 1
L 9.0 10 3 100

MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

Notes:

  1. For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
  2. For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
  3. All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.

Specifications

What's in the box?

Item Part # Size

MULTI-CORE™ 10X Buffer

R999B 3 × 1ml

SDS

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Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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