CRISPR/Cas9 technology has revolutionized genome editing by offering a simple method to tag proteins at endogenous loci, facilitating the study of protein biology while maintaining proper transcriptional regulation, expression levels and stoichiometry with binding partners. By contrast, ectopic expression of tagged proteins can lead to a variety of overexpression artifacts, like mislocalization, aggregation or dysregulation of degradation. HiBiT, an 11-amino-acid bioluminescent peptide, represents an ideal tag for endogenous labeling due to its small size and large, linear dynamic range.
In this webinar, we will highlight an efficient, cloning-free method for knock-in of HiBiT, as well as other protein tags, and we will discuss how these endogenously modified cells can be used in a variety of assay formats to study protein abundance, localization, modification and interactions. Additionally, we will discuss do-it-yourself approaches, the availability of pools and stable clones, and support for assay development.
Christopher Eggers, PhD
Senior Research Scientist
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