Nano-Glo Dual-Luciferase Reporter Assay Protocol
Literature # TM426
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System allows sensitive detection of both firefly luciferase and NanoLuc® luciferase in the same sample. The add-read-add-read, or “Stop & Glo”, format is amenable to assays or screens in 96-, 384- or 1,536-well plates. Firefly luciferase activity is measured first using the supplied ONE-Glo™ EX Luciferase Assay Reagent, NanoDLR™ Stop & Glo® Reagent is then added to quench the firefly signal (>1,000,000-fold) and to provide the substrate needed to measure NanoLuc® luciferase activity. Potent inhibition of firefly luciferase coupled with the high-intensity luminescence of NanoLuc® luciferase maximizes sensitivity for detection of both reporters and allows either one, or both, to be used to measure a biological response.