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HisLink™ Protein Purification Resin

Resin to Purify His- or HQ-Tagged Protein from Cleared Lysate

  • Purify polyhistidine- or HQ-tagged proteins from cleared lysate by standard column chromatography or from crude lysate in batch
  • Suitable for low- to medium-pressure applications such as FPLC
  • Large quantities of polyhistidine- or HQ-tagged protein can be purified per volume of resin used

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Catalog number selected: V8821

$ 312.00
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HisLink™ Protein Purification Resin
50ml
$ 312.00
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HisLink™ Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin) for efficient capture and  purification of bacterially expressed polyhistidine- or HQ-tagged proteins.

HisLink™ Resin may be used in either column or batch purification formats. Gravity feed of a cleared lysate over a HisLink™ column is sufficient for complete capture and efficient elution of polyhistidine- or HQ-tagged proteins; however, the resin also may be used with vacuum filtration devices (e.g., the Vac-Man® Vacuum Manifold, Cat.# A7231) to allow simultaneous processing of multiple columns.

HisLink™ Resin also is an excellent choice for affinity purification using low- to medium-pressure liquid chromatography systems such as fast performance liquid chromatography (FPLC). In batch format, HisLink™ Resin may be easily separated from the lysate without filtration, allowing processing of larger quantities of lysate and purification of protein from lysate that has not been cleared of insoluble cellular debris.

Note: Resin product volumes represent total volume of a 50% resin slurry.

Specifications

You are viewing: V8821 Change Configuration

What's in the box?

Item Part # Size

HisLink™ Protein Purification Resin

V882A 1 × 50ml

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

Specifications

You are viewing: V8823 Change Configuration

What's in the box?

Item Part # Size

HisLink™ Protein Purification Resin

V882C 1 × 5ml

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

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