DNA Ladders have defined sizes, and are not intended for use in quantitative analysis.
The 1kb DNA Ladder has thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels for easy identification. All other fragments are of equal intensity. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase for visualization by autoradiography.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 1mM EDTA.
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