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UDP-Glo™ Glycosyltransferase Assay

Universal Glycosyltransferase Activity Assay

  • Easy, "add-incubate-read" protocol
  • Detects any glycosyltransferase that uses UDP-sugar as a substrate
  • Ideal for low-activity glycosyltransferases

Size

Add a UDP-sugar substrate

Catalog number selected: V6961

$ 330.00
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This product is discontinued
UDP-Glo™ Glycosyltransferase Assay
200 assays
$ 330.00
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A Simple, Bioluminescent Assay for Glycosyltransferase Activity

The UDP-Glo™ Glycosyltransferase Assay provides a homogeneous, single-reagent-addition method to rapidly detect UDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of UDP Detection Reagent is added to convert the UDP product to ATP and generate light in a luciferase reaction. The light generated is correlated to UDP concentration by using an UDP standard curve.

This assay is intended for use with purified glycosyltransferases that use UDP-sugar as a donor substrate and cannot be used with whole cells or cell extracts. However, glycosyltransferases purified from cell extracts using immunoprecipitation or affinity tag pulldown can be used in the UDP-Glo™ Assay.

Applications of the UDP-Glo Assay include profilng glycosyltransferase specificity for different sugars, screening library compounds for effects on glycosyltransferase activity, and detecting chemical compound glucuranidation during drug discovery.

12347MA-W
UDP-Glo™ Glycosyltransferase Assay Reaction: Following the glycosyltransferase reaction, free UDP is converted into ATP, which is then converted into a luminescent signal that is proportional to the glycosyltransferase activity in the reaction.

Works with Most Glycosyltransferases

UDP-Glo™ can be used to measure the activity of just about any glycosyltransferase that uses a UDP-sugar as a substrate. Here, we looked at GTs involved in glycan biosynthesis, post-translational modification and drug metabolism.
12348MB-panelA
12348MB-panelB
12348MB-panelC
12348MB-panelD

Detects Low Nanomolar Amounts of UDP; Ideal for Low-Activity Glycosyltransferases

The assay response is linear in the nanomolar to micromolar range, allowing use of low concentrations of UDP-sugar, and alleviating issues due to feedback glycosyltransferase inhibition.

The data to the left shows the linearity and sensitivity of the UDP-Glo™ Glycosyltransferase Assay. Here, a UDP standard curve was prepared over the indicated range of UDP concentrations in 25µl of 1X glycosyltransferase reaction buffer in a solid white 96-well plate. UDP-Glo™ Glycosyltransferase Assay was performed using 25µl of UDP Detection Reagent at room temperature. Luminescence was recorded using a GloMax® luminometer. Values represent the mean of four replicates. The table shows luminescence measured 1 hour after adding the UDP Detection Reagent, and signal-to-background ratios calculated for each concentration of the UDP standard curve.
Standard curve using UDP concentrations from 0.02 micromolar to 25 micromolar.

Luminescent Signal is Stable Over Several Hours

The UDP-Glo™ Assay  generates a highly stable luminescent signal with >80% signal remaining after 3 hours, allowing convenient batch-processing of assay plates.

The data here shows that the signal obtained with the UDP-Glo™ Assay is stable for several hours. To determine signal stability, luminescence was recorded every hour. The signal-to-background ratio did not change over the time measured (data not shown). RLU = relative light units.

12346MA-W-c

Specifications

You are viewing: V6961 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (175 KB) – English (United States)

Certificate of Analysis

See all certificates for V6961

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6962 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP, 10mM

V698A 1 × 100μl

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (175 KB) – English (United States)

Certificate of Analysis

See all certificates for V6962

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6963 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363B 1 × 1 vial

Nucleotide Detection Buffer

V696C 1 × 100ml

UDP, 10mM

V698A 1 × 100μl

UDP-Glo™ Enzyme

V697C 1 × 200μl

Enzyme Dilution Buffer

V773B 1 × 15ml

SDS

Choose language:

Download SDSPDF (175 KB) – English (United States)

Certificate of Analysis

See all certificates for V6963

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6971 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

UDP-GlcNAc, 100mM

V707A 1 × 50μl View Product

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6971

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6972 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

UDP, 10mM

V698A 1 × 100μl

UDP-GlcNAc, 100mM

V707A 1 × 50μl View Product

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6972

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6981 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl

UDP-GalNAc, 100mM

V708A 1 × 50μl View Product

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6981

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6982 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

UDP, 10mM

V698A 1 × 100μl

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP-GalNAc, 100mM

V708A 1 × 50μl View Product

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6982

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6991 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl

UDP-Glc, 100mM

V709A 1 × 50μl View Product

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6991

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V6992 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP, 10mM

V698A 1 × 100μl

UDP-Glc, 100mM

V709A 1 × 50μl View Product

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V6992

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V7051 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl
UDP-Gal, 100mM V717A 1 × 50μl View Product

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V7051

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V7052 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP, 10mM

V698A 1 × 100μl
UDP-Gal, 100mM V717A 1 × 50μl View Product

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V7052

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V7061 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

UDP-Glo™ Enzyme

V697A 1 × 20μl

UDP, 10mM

V698A 1 × 100μl

UDP-GA, 100mM

V732A 1 × 50μl View Product

Enzyme Dilution Buffer

V773A 1 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V7061

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

Specifications

You are viewing: V7062 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

UDP, 10mM

V698A 1 × 100μl

UDP-GA, 100mM

V732A 1 × 50μl View Product

UDP-Glo™ Enzyme

V697B 1 × 40μl

Enzyme Dilution Buffer

V773A 2 × 1.5ml

SDS

Choose language:

Download SDSPDF (199 KB) – English (United States)

Certificate of Analysis

See all certificates for V7062

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

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