The UDP-Glo™ Glycosyltransferase Assay provides a homogeneous, single-reagent-addition method to rapidly detect UDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of UDP Detection Reagent is added to convert the UDP product to ATP and generate light in a luciferase reaction. The light generated is correlated to UDP concentration by using an UDP standard curve.
This assay is intended for use with purified glycosyltransferases that use UDP-sugar as a donor substrate and cannot be used with whole cells or cell extracts. However, glycosyltransferases purified from cell extracts using immunoprecipitation or affinity tag pulldown can be used in the UDP-Glo™ Assay.
Applications of the UDP-Glo Assay include profilng glycosyltransferase specificity for different sugars, screening library compounds for effects on glycosyltransferase activity, and detecting chemical compound glucuranidation during drug discovery.