The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The SPHK2 Kinase Enzyme System contains:
- SPHK2 Kinase, 10μg (Human, recombinant, full-length); MW: ~110kDa
- Sphingosine Substrate
- 5X Reaction Buffer; DTT, 0.1M
Recombinant full-length human SPHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PHK2 is a member of the SPHK family and catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). Sphingolipid metabolism regulates proper uterine decidualization and blood vessel stability. SPHK2 is involved in the regulation of chemosensitivity by controlling ceramide formation and the downstream Akt pathway in human colon cancer cells. SPHK2 plays an important role in migration of MDA-MB-453 cells toward the ligand EGF.
NCBI Database Entry
The SPHK2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.