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SignaTECT® Protein Kinase Assay Systems

V8161_SignaTECT--CaMKII-Protein-Kinase-Assay_3
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Radioactive Assays for Detection of Kinase Activity

  • Assays for calcium/calmodulin-dependent protein kinase

Choose a kinase assay

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Catalog number selected: V8161

$ 551.00
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SignaTECT® Protein Kinase Assay Systems
CaMKII/96 reactions
$ 551.00
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Convenient, 1-Hour Kinase Activity Assays

The SignaTECT® Protein Kinase Assay Systems provide a rapid, radioactivity-based method for detection of kinase activity. The method is based on capture of phosphorylated labeled substrates on the SAM2® Biotin Capture Membrane, which offers low background and high signal-to-noise ratios, even for complex samples such as crude cell extracts, while retaining the high substrate capacity necessary to maintain optimum reaction kinetics.

Each SignaTECT® System contains a highly specific biotinylated peptide substrate for the appropriate kinase as well as the necessary reaction components. The researcher must supply [γ-32P]ATP.

Method Overview

After the kinase reaction is complete, the biotinylated, 32P-labeled substrate is recovered from the reaction mix using the SAM2® Biotin Capture Membrane. After samples are spotted onto the SAM2® Membrane a series of short washes are performed to remove nonspecific label. The process is complete in less than 1 hour. The SAM2® Membrane is prenumbered and partially cut so that individual squares can be easily identified, separated and placed in scintillation vials. Alternatively, the intact SAM2® Membrane can be analyzed by phosphorimaging or conventional autoradiography.

Advantages of the SAM2® Membranes

  • High binding capacity and high specificity characteristics produce lower backgrounds and higher signal-to-noise ratios compared to the traditional P81 phosphocellulose method of capture and measurement.
  • The perforated and numbered membrane allows researchers to measure from 1 up to 96 kinase reactions and does not require as much "hands-on" manipulation as other methods used to measure kinase activity.
  • Can be used under a variety of buffer/reaction conditions (e.g., cell extracts), which many other methods do not allow.
  • High binding capacity allows use of the SignaTECT® Systems for kinetic studies.

Specifications

You are viewing: V7470 Change Configuration

What's in the box?

Item Part # SizeConcentration

ATP

V222A 1 × 600μl0.5mM

PKC Activation 5X Buffer

V226A 3 × 200μl

Control 5X Buffer

V228A 1 × 600μl

BSA

V275A 1 × 0.2ml10mg/ml

SAM2® Biotin Capture Membrane

V2861 1 × 96 samples

PKC Coactivation 5X Buffer

V592A 1 × 600μl

PKC Biotinylated Peptide Substrate

V593A 1 × 600μl0.5mM

Termination Buffer

V594A 2 × 700μl

SDS

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Download SDSPDF (350 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: V6480 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

DTT

P117A 1 × 100μl100mM View Product

ATP

V222A 1 × 600μl0.5mM

Trichloroacetic Acid (TCA), 43%

V271A 1 × 400μl

TCA Neutralization 5X Buffer

V272A 1 × 600μl

BSA

V275A 1 × 0.2ml10mg/ml

SAM2® Biotin Capture Membrane

V2861 1 × 96 samples

PTK Biotinylated Peptide Substrate 1

V287A 1 × 240μl2.5mM

PTK Biotinylated Peptide Substrate 2

V288A 1 × 240μl2.5mM

Termination Buffer

V594A 2 × 700μl

PTK Assay 5X Buffer

V649A 1 × 500μl

PTK Enzyme Dilution 5X Buffer

V651A 1 × 200μl

Sodium Vanadate

V652A 1 × 250μl1mM

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: V8161 Change Configuration

What's in the box?

Item Part # SizeConcentration

ATP

V222A 1 × 600μl0.5mM

BSA

V275A 1 × 0.2ml10mg/ml

SAM2® Biotin Capture Membrane

V2861 1 × 96 samples

Termination Buffer

V594A 2 × 700μl

CaM KII Biotinylated Peptide Substrate

V817A 1 × 300μl

CaM KII Reaction 5X Buffer

V818A 1 × 1ml

CaM KII Control 5X Buffer

V819A 1 × 500μl

CaM KII Activation 5X Buffer

V820A 1 × 500μl

SDS

Choose language:

Download SDSPDF (374 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 6,066,462, 6,348,310 and 6,753,157.

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