Kinase Selectivity Profiling Systems—AGC
Selectivity Screening of Library Compounds for Effect on AGC Family Kinases
- Kinase and substrate pairs organized in an easy-to-use 8-tube strip format
- Simple reaction assembly and automation-friendly protocol
- Streamlines profiling of hits and leads for potency against families of related kinases
Catalog Number:
Choose a kinase profiling system
Additional options
Catalog Number: V6858
Catalog Number: V6859
Catalog Number: V6910
Catalog Number: V6931
Profile Lead Compounds in Hours, Not Days
The Kinase Selectivity Profiling Systems are ideal for profiling lead-compound kinase inhibitors against a broad panel of kinases to better understand inhibitor activity and obviate any off-target effects. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
The AGC1 and AGC2 Kinase Selectivity Profiling Systems contain sets of kinases from the AGC subfamily of protein kinases. Each profiling system contains the reagents needed to complete the profile, including kinase reaction buffer, eight kinases in each multiwell strip and eight corresponding substrates and cofactors in another multiwell strip.
Optimized for Use With ADP-Gloâ„¢ Kinase Assay
The Kinase Selectivity Profiling Systems rely on the luminescent ADP-Gloâ„¢ Kinase Assay technology to characterize kinase selectivity. The luminescence generated by the ADP-Gloâ„¢ Kinase Assay is proportional to ADP concentration and correlates with kinase activity.
Each Kinase Selectivity Profiling System is available with or without ADP-Gloâ„¢ Assay reagents.
Simple, Automation-Friendly Assay, No Time-Consuming Optimization
Simply dilute the stock solutions provided, and you’re ready to go.  Two quick, one-step dilutions provide working stocks of kinases and substrate/cofactor solutions.Â
Concentrated kinases and substrates/cofactor stocks are first diluted and then combined with test compound in a 384-well plate. After a 1-hour incubation, the ADP-Gloâ„¢ Assay is performed to measure kinase activity.
Assays can be performed manually, or automated on a liquid-handler.
Single-Dose Profiling
Specifications
Catalog Number:
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Kinase Selectivity Profiling System: AGC-1 |
V6858 | 1 × 8 × 50 reactions |
ADP-Glo™ Kinase Assay |
V6930 | 1 × 400 assays |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
U.S. Pat. No. 7,700,310 and other patents and patents pending.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Kinase Selectivity Profiling System: AGC-2 |
V6910 | 1 × 8 × 50 reactions |
ADP-Glo™ Kinase Assay |
V6930 | 1 × 400 assays |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
U.S. Pat. No. 7,700,310 and other patents and patents pending.
Resources
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Frequently Used With
ADP-Gloâ„¢ Kinase Assay
Universal, luminescent kinase activity assay.
V6930, V9101, V9102, V9103, V9104
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
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Dedicated microplate luminometer.
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