We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

M-MLV Reverse Transcriptase

Don't see what you are looking for?

Click here for custom options.

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Expand to Read More »

  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price Your Price QTY Add to Cart

M-MLV Reverse Transcriptase

10,000u
200 u/μl M1701 $ 64.00 $ 60.80 Add to cart

M-MLV Reverse Transcriptase

50,000u
200 u/μl M1705 $ 244.00 $ 231.80 Add to cart

M-MLV Reverse Transcriptase Buffer Pack

2 × 1ml
- M5313 $ 18.00 $ 17.10 Add to cart


QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.

For product intended use please see Patents & Disclaimers tab.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.