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M-MLV Reverse Transcriptase

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Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

M-MLV Reverse Transcriptase

10,000u
200u/μl M1701 $ 66.00 Add to cart

M-MLV Reverse Transcriptase

50,000u
200u/μl M1705 $ 254.00 Add to cart

M-MLV Reverse Transcriptase Buffer Pack

2 × 1ml
- M5313 $ 18.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.

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