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Promega Corporation

M-MLV Reverse Transcriptase

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Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

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M-MLV Reverse Transcriptase

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M-MLV Reverse Transcriptase

 Components

  • M-MLV Reverse Transcriptase

    M170A1 x 10,000u
  • M-MLV RT 5X Buffer

    M531A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10,000u
200u/μl M1701 $ 64.00 Add to cart

M-MLV Reverse Transcriptase

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M-MLV Reverse Transcriptase

 Components

  • M-MLV Reverse Transcriptase

    M170B1 x 50,000u
  • M-MLV RT 5X Buffer

    M531A2 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50,000u
200u/μl M1705 $ 244.00 Add to cart

M-MLV Reverse Transcriptase Buffer Pack

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M-MLV Reverse Transcriptase Buffer Pack

 Components

  • M-MLV RT 5X Buffer

    M531A2 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


2 × 1ml
- M5313 $ 18.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.

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