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Promega Corporation

RT-PCR

Learn about RNA Analysis Tools

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RT-PCR
Reverse Transcriptases and RT-PCR Kits

Choose from a wide range of reverse transcriptases and RT-PCR kits for robust, convenient reverse transcription of full-length cDNA from abundant and rare mRNAs. From M-MLV enzymes for routine RT-PCR to GoScript™ Reverse Transcriptase for sensitive RT-qPCR.

Featured Products

GoScript™ Reverse Transcription System

Produce multiple cDNAs using oligo(dT)15 or random primers during first-strand cDNA synthesis and/or divide into multiple second-strand cDNA syntheses and PCR amplifications with different gene-specific primers.

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

A cost-effective, quality reverse transcriptase for routine RT-PCR and cDNA synthesis.

AccessQuick™ RT-PCR System

Saves you four pipetting steps by combining several components into a master mix.

AMV Reverse Transcriptase

Great for RT-PCR templates with high secondary structure.

Featured Resources

pubhub_fr Choosing the Right Reverse Transcriptase

Important considerations for choosing a reverse transcriptase, such as the downstream application, length of the target RNA, presence of complex RNA secondary structure, and the enzyme's level of RNase H activity.

protocols_fr GoTaq® 2-Step RT-qPCR System Technical Manual

A protocol for quantitative analysis of RNA using two-step RT-qPCR. The system provides reagents for cDNA synthesis using the GoScript™ System and quantification using the GoTaq® qPCR Master Mix.

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