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Designing a Bioluminescent Reporter Assay: Normalization

Comparison of Glucose Uptake Assay Methods

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LabFact #35

When expressing your protein in a cell-free system, make sure the combination of promoter and DNA conformation is appropriate. For example, the T7 RNA polymerase promoter may express better in a linearized template while the SP6 promoter transcribes better in a circular DNA.

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by Ed Himelblau

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