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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

RNA Extraction from Formalin Fixed Paraffin Embedded (FFPE) Tissue Enabling Next Generation Detection of Gene Fusions

MDx

Tuesday, January 24, 2017

Dr. William Rehrauer
University Wisconsin-Madison

Learn about the detection of novel RNA fusions via new next generation sequencing library prep methods, automated extraction of total nucleic acid and the utility of this research in the field of molecular oncology.

To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

Genomics

Tuesday, February 14, 2017

Doug Wieczorek, PhD

Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

How to Interrogate Epigenetic Events in Live Cells

Cell-Based Assays

Tuesday, January 10, 2017

Danette L Daniels, PhD

Modification of gene expression rather than alteration of the genetic code (Epigenetics) is an interesting way that organisms develop a phenotype. Altered expression of the key proteins can have deleterious effects. Researchers are actively engaged in identifying modulators of epigenetic proteins and learning how these proteins interact with their targets. Learn more as we present data on the use of a novel BRET assay for such studies.

Results from a Casework Pilot Study for PowerPlex® Y23 System

Genetic Identity

Monday, December 19, 2016

Mr. Jim Thomson

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples. In this webinar, Jim Thomson from LGC Group described the use of PowerPlex® Y23 System on difficult sexual offense cases, particularly those in which no sperm was identified. He shared success rates on different categories derived from a case study carried out on samples provided by nominated participating UK police forces.

Guidelines for Developing Robust and Reliable PCR Assays

MDx

Tuesday, December 06, 2016

Leta Steffen, PhD

Don't settle for a mediocre PCR assay. Learn how experts develop robust and reliably performing PCR assays.

The Key Role of FcR Activating Antibodies in Generating "Sterilizing" Immunity Against Herpes Simplex Virus

Drug Discovery

Tuesday, November 08, 2016

Betsy Herold, MD

Antibody dependent cell mediated cytotoxicity (ADCC) is an important immune effector mechanism against tumor and virus-infected cells. In recent years, ADCC has emerged as an important protective immune correlate in a number of vaccine studies for HIV and influenza. This webinar will highlight recent studies demonstrating the central role of FcR functions in vaccine-mediated protection against HSV-1 and HSV-2 infection, challenging the existing paradigm that high titer neutralizing antibodies are required.

Faster Mass Spec: Same-Day Sample Prep Now a Reality

Proteomics

Tuesday, October 25, 2016

Micheal M. Rosenblatt, PhD

Traditional protocols require digestion times of up to 18 hours, as well as reduction and alkylation prior to digestion. These denaturants often inhibit enzymes and modify proteins, and require removal prior to analysis. What if you could shorten digestion times to ~30 minutes, without denaturants or reductants or alkylating agents?

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