BCAA-Glo™ Assay

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Easy, Sensitive Branched-Chain Amino Acid (Leu/Ile/Val) Assay Kit

  • Compatible with cells, media, tissue and serum samples
  • In-well sample preparation protocol streamlines workflow
  • Scalable to 384-well plates for HTS applications

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Catalog number selected: JE9300

$ 475.00
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BCAA-Glo™ Assay
5ml
$ 475.00
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Efficient BCAA Detection from Diverse Samples

The BCAA-Glo™ Assay is a fast and sensitive method for detecting branched-chain amino acids (BCAA; leucine, isoleucine and valine) from various biological samples, such as cultured cells, media, tissue homogenates and serum. Based on bioluminescent technology, the BCAA-Glo™ Assay can detect subtle changes in amino acid metabolism and abundance. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.

How the BCAA-Glo™ Assay Works

Diagram illustrating how the BCAA-Glo™ Assay works.

Leucine Dehydrogenase catalyzes the oxidation of BCAA, simultaneously reducing NAD+ to NADH. In the presence of NADH, Reductase converts the proluciferin Reductase Substrate into luciferin. This reaction is detected by Ultra-Glo™ Recombinant Luciferase, which emits light proportionate to the amount of BCAA in the sample.

Leucine Dehydrogenase recognizes all three BCAA with similar efficiency. It is specific for BCAA L-isomers.

Simple Add-and-Read Protocol Saves Time

Diagram illustrating the BCAA-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in Branched-Chain Amino Acids

Graph showing the linear relationship between luminescence and leucine concentration.

 

Sensitivity (S/B >5)

400nM

Limit of Detection (S/N >3)

50nM

Linear Range

50nM to 25μM

Maximum Assay Window (S/B)

>300

Sensitive Quantitation of BCAA in Cultured Cells

Plot showing the linear relationship between luminescence and HCT116 and SKOV-3 cell lysate samples as measured by the BCAA-Glo™ Assay.

Cellular BCAA levels vary depending on cell type, growth conditions and metabolic activity. Intracellular BCAA concentrations are typically low. However, the BCAA-Glo™ Assay, with its high sensitivity and wide linear detection range, successfully measures BCAA levels in cell lysate samples ranging from 5,000 to 40,000 cells per 50μl, as shown in the graph.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

Specifications

You are viewing: JE9300 Change Configuration

What's in the box?

Item Part # Size Concentration

Leucine Dehydrogenase

J412A 1 × 100μl

Leucine, 10mM

J413A 1 × 50μl

Reductase

G884A 1 × 55μl 6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Solution

J135A 1 × 5ml

NAD

J136B 1 × 275μl

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154B 1 × 100μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Specifications

You are viewing: JE9400 Change Configuration

What's in the box?

Item Part # Size Concentration

Leucine Dehydrogenase

J412B 1 × 1ml

Leucine, 10mM

J413A 1 × 50μl

Reductase

G884B 1 × 275μl 6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Solution

J135B 1 × 50ml

NAD

J136C 1 × 1ml

Neutralization Buffer

J153A 1 × 15ml

NADP+

J154C 1 × 500μl

0.6N HCl

J402A 1 × 15ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

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Uwe

Germany