As the antibody therapeutics industry expands, new methods are being sought to improve the efficiency of lead antibody identification in the early discovery phase. A key step in this direction is the ability to assay large numbers of test antibodies in multiple biologically relevant assays. Some antibody assays, such as enzyme-linked immunosorbent assays (ELISAs) for binding to antigen, can be performed on antibodies in cell media. However, many functional assays, especially cell-based assays, require highly pure antibodies at high concentrations.
We have developed high-capacity, magnetic Protein A and Protein G beads for antibody purification (Magne® Protein A Beads, Cat.# G8781; Magne® Protein G Beads, Cat.# G7471) that are compatible with automated platforms. In this report we demonstrate the KingFisher® Flex platform for automated 96-well processing of magnetic particles for parallel purification of eight different mouse and human antibodies using Magne® Protein A and Magne® Protein G Beads. To demonstrate the reproducibility of the purification process, six replicates of each antibody were purified.