CellTiter-Glo® 2.0 Assay Technical Manual

Instructions for Use of Product(s)
G9241, G9242, G9243

Literature # TM403

The CellTiter-Glo® 2.0 Assay provides a homogeneous method to determine the number of viable cells in culture by quantitating the amount of ATP present, which indicates the presence of metabolically active cells. This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. Although similar to the CellTiter-Glo® One Solution Assay, which needs to be stored frozen, the CellTiter-Glo® 2.0 Assay is much more stable and will maintain > 85% light output upon storage at 4°C for 2 months or >85% light output upon storage at room temperature for 7 days. Functional performance (linearity, sensitivity and signal half-life) of the product is maintained at these temperatures for longer periods, although light output will decline over time. The CellTiter-Glo® 2.0 Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays.

Summary of Changes
The following changes were made to the 1/23 revision of this document:
1. Related Products and patent statements were updated.
2. Cover image and font were updated.

Revised 1/23

Experienced User Protocols