Starting a new lab? Save 50% when you register for our New Lab Startup Program. Sign Up Today ›

pCI-neo Mammalian Expression Vector

E1841_pCI-neo-Mammalian-Expression-Vector-20ug_3

Constitutively Express Cloned Insert for Transient or Stable Transfection

  • Select for stably transfected cells with neomycin phosphotransferase gene
  • Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
  • Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication

Size

Catalog number selected: E1841

$ 450.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
pCI-neo Mammalian Expression Vector
20μg
$ 450.00
Your price: Log in

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.

References

  1. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
  2. Choi, T. et al. (1991) Mol. Cell. Biol. 11, 3070–4.
  3. Palmiter, R.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 478–82.
  4. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.
0914VA01_5A-W

pCI-neo Mammalian Expression Vector GenBank® Accession Number U47120 and vector sequence text file.

Specifications

What's in the box?

Item Part # Size

pCI-neo Mammalian Expression Vector

E184A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

Luca

Luca

Italy