The PowerPlex® 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. This system allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane.
The PowerPlex® 16 System has undergone extensive validation efforts including internal validation, CODIS database validation and forensic casework validation. This system is optimized for use with 1ng of DNA; however, each lot performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with <0.5ng of DNA. The Internal Lane Standard 500 marker is used in each gel lane or capillary injection to increase the precision of analysis and allelic ladders for all 16 loci are provided to simplify interpretation. Panels and bins text files are required for use with GeneMapper® ID and GeneMapper® ID-X software and are available for download.
The PowerPlex® 16 System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130 and 3130xl Genetic Analyzers. The PowerPlex® Matrix Standards, 310 (Cat.# DG4640), is required for use of this system with the ABI PRISM® 310 Genetic Analyzer. The PowerPlex® 4C Matrix Standard (Cat.# DG4800) is required for use of this system with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers.